Contents

1. Expression strategy (Michael Dyson)

2. Protein expression in Escherichia coli (Rosalind Kim)

3. Expression engineering of synthetic antibodies using ribosome display (Matthew DeLisa and Lydia M. Contreras Martinez)

4. Refolding proteins from inclusion bodies (Renaud Vincentelli)

5. Selection of protein variants with improved expression using GFP-derived folding and solubility reporters (Geoffrey Waldo and St 'e9phanie Cabantous)

6. Protein expression in the wheat germ cell-free system (Yaeta Endo and Tatsuya Sawasaki)

7. Saccharomyces cerevisiae ; A microbial eukaryotic expression system (Christine Lang)

8. Expression of proteins in Pichia pastoris (Geoff and Joan Lin-Cereghino and Wilson Leung)

9. Improved baculovirus expression vectors (Linda King, Richard Hitchman and Robert Possee)

10. Transient transfection of insect cells for rapid expression screening and protein production (Robert Novy et al.)

11. Generation of stable CHO cell lines for protein expression (Zhijian Lu et al.)

12. Transient expression in HEK293-EBNA1 cells (Yves Durocher, Roseanne Tom and Louis Bisson)

13. Nisin- and subtilin-controlled gene expression systems for Gram-positive bacteria (Oscar Kuipers and Jan Kok)

14. Protein expression using lentiviral vectors (Bernard Massie, Renald Gilbert and Sophie Broussau)

15. Expression in mammalian cells using BacMam viruses (Yu-Chen Hu and Hsiao-Ping Lee)

List of suppliers

Index