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From Genes to Genomes 2/e : Concepts and Applications of DNA Technology
ÆǸŰ¡°Ý  : 35,000¿ø
Àû¸³±Ý  : 1,050Á¡
ÃâÆǻ砠: Wiley
ÀúÀÚ  : Dale
¹ßÇàÀÏ  : 2007³â
ÆäÀÌÁö ¼ö  : 394¸é
ISBN  : 9780470017340
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Preface
1 Introduction

2 Basic molecular biology

2.1 Nucleic acid structure

2.2 What is a gene?

2.3 Information flow: gene expression

2.4 Gene structure and organization

3 How to clone a gene

3.1 What is cloning?

3.2 Overview of the procedures

3.3 Gene libraries

3.4 Hybridization

3.5 Polymerase chain reaction

3.6 Extraction and purification of nucleic acids

3.7 Detection and quantitation of nucleic acids

3.8 Gel electrophoresis

4 Cutting and joining DNA

4.1 Restriction endonucleases

4.2 Ligation

4.3 Modification of restriction fragment ends

4.4 Other ways of joining DNA molecules

5 Vectors

5.1 Plasmid vectors

5.2 Vectors based on the lambda bacteriophage

5.3 Cosmids

5.4 M13 vectors


CONTENTS

5.5 Expression vectors

5.6 Vectors for cloning and expression in eukaryotic cells

5.7 Supervectors: YACs and BACs

5.8 Summary

6 Genomic and cDNA libraries

6.1 Genomic libraries

6.2 Growing and storing libraries

6.3 cDNA libraries

6.4 Random, arrayed and ordered libraries

7 Finding the right clone

7.1 Screening libraries with gene probes

7.2 Screening expression libraries with antibodies

7.3 Subcloning

7.4 Characterization of plasmid clones

8 Polymerase chain reaction

8.1 The PCR reaction

8.2 PCR in practice

8.3 Cloning PCR products

8.4 Long-range PCR

8.5 Reverse-transcription PCR

8.6 Rapid amplification of cDNA ends

8.7 Quantitative PCR

8.8 Applications of PCR

9 Characterization of a cloned gene

9.1 DNA sequencing

9.2 Databank entries and annotation

9.3 Sequence analysis

9.4 Sequence comparisons

9.5 Protein structure 

9.6 Confirming gene function

10 Analysis of gene expression

10.1 Analysing transcription 

10.2 Methods for studying the promoter 

10.3 Regulatory elements and DNA-binding proteins

10.4 Translational analysis


CONTENTS

11 Products from native and manipulated cloned genes 

11.1 Factors affecting expression of cloned genes

11.2 Expression of cloned genes in bacteria

11.3 Expression in eukaryotic host cells

11.4 Adding tags and signals

11.5 In vitro mutagenesis

11.6 Vaccines

12 Genomic analysis

12.1 Genome sequencing

12.2 Analysis and annotation 

12.3 Comparing genomes 

12.4 Genome browsers

12.5 Relating genes and functions: genetic and physical maps

12.6 Transposon mutagenesis and other screening techniques

12.7 Conclusion

13 Analysis of genetic variation

13.1 Single nucleotide polymorphisms

13.2 Larger-scale variations 

13.3 Other methods for studying variation

13.4 Human genetic diseases

13.5 Molecular phylogeny

14 Post-genomic analysis

14.1 Analysing transcription; transcriptomes

14.2 Array-based methods

14.3 Translational analysis; proteomics 3

14.4 Post-translational analysis: protein interactions

14.5 Integrative studies; systems biology

15 Modifying organisms; transgenics

15.1 Modification of bacteria and viruses: live vaccines

15.2 Transgenesis and cloning

15.3 Animal transgenesis

15.4 Applications of transgenic animals

15.5 Transgenic plants and their applications

Glossary

Bibliography

Index


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