Part 1: Gene Identification1 Maarten Koornneef: Classical mutagenesis in higher plants2 Karabi Datta and Swapan K Datta: Plant transfomrmation3 Csaba Koncz and Jeff Schell: T-DNA tagging4 Andy Pereira: Transposion tagging methods in heterologous plants5 Tai-ping Sun: Cloning plant genes by genomic subtractionPart 2: Gene Organization6 S Grandillo and T M Fulton: Approaches to gene mapping7 Paul Muskett, Ian Bancroft and Caroline Dean: Arabidopsis YAC, BAC and cosmid libraries8 J S Heslop-Harrison and Trude Schwarzacher: In situ hybridization to plant chromosomes and DNA fibresPart 3: Library Screening and cDNA Isolation9 Michiel Vandenbussche and Tom Gerats: PCR cloning approaches10 Rhonda C Foley and Karam B Singh: Western and southwestern library screens11 E Ann Oakenfull and James A H Murray: Complementation Cloning