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ISBN  : 978-89-5881-275-3
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½ÇÇè 1 ¹ÚÅ׸®¾Æ ¹è¾ç ¹èÁö 32
½ÇÇè 2 Pure ¹ÚÅ׸®¾ÆÀÇ ºÐ¸® ¹× ¹è¾ç 41
½ÇÇè 3 ¼¼±Õ ¼öÀÇ ÃøÁ¤(ÆòÆÇ °è¼ö¹ý, Plate count technique) 47
½ÇÇè 4 ¹ÚÅ׸®¾Æ ¼¼Æ÷ÀÇ ¼ºÀå ¹× ±ÕÁÖ(strain) º¸°ü¹ý 53
½ÇÇè 5 Á¢ÇÕ(conjugation) 61
½ÇÇè 6 ¹ÚÅ׸®¾Æ ¼¼Æ÷·ÎºÎÅÍ plasmid DNAÀÇ extraction°ú purification 64
½ÇÇè 7 ÇÙ»ê°ú ´Ü¹éÁúÀÇ ³óµµ ÃøÁ¤ 73
½ÇÇè 8 Agarose gel electrophoresis 77
½ÇÇè 9 Agarose gel·ÎºÎÅÍ fragmented DNAÀÇ recovery ¹× Á¤Á¦ 83
½ÇÇè 10 Plasmid vector¸¦ ÀÌ¿ëÇÑ subcloning 87
½ÇÇè 11 Recombinant Plasmid DNAÀÇ ÇüÁúÀüȯ 94
½ÇÇè 12 ÇüÁúÀüȯüÀÇ °íµî»ý¹°Ã¼·ÎÀÇ ÇüÁúÀüȯ ¹× ºÐ¼® 100
½ÇÇè 13 ¥â-galactosidase enzymeÀÇ induction 113
½ÇÇè 14 Recombinant plasmid DNAÀÇ Å½Áö¿Í Á¦ÇÑÈ¿¼Ò Áöµµ ÀÛ¼º 117
½ÇÇè 15 ÁøÇÙ»ý¹°·ÎºÎÅÍ Genome DNAÀÇ ÃßÃâ 123
½ÇÇè 16 Eukaryotic DNAÀÇ partial restriction enzyme digestion 127
½ÇÇè 17 Southern Hybridization 130
½ÇÇè 18 In situ hybridization 136
½ÇÇè 19 ¹ýÀÇÇÐÀû Ãø¸é¿¡¼­ÀÇ fingerprinting 143
½ÇÇè 20 ÁßÇÕÈ¿¼Ò ¿¬¼â¹ÝÀÀ(PCR, Polymerase Chain Reaction) 151
½ÇÇè 21 UV¿¡ ÀÇÇÑ DNA ¼Õ»ó ¹× ±¤È¸º¹ 155
½ÇÇè 22 °íµî»ý¹° Á¶Á÷À¸·ÎºÎÅÍ total RNAÀÇ extraction 159
½ÇÇè 23 mRNA(poly(A)+)ÀÇ Purification and selection 168
½ÇÇè 24 Northern hybridization 172
½ÇÇè 25 Dot and slot hybridization of RNA 180
½ÇÇè 26 Tyrosinase ´Ü¹éÁúÀÇ ÃßÃâ ¹× Á¤Á¦ 185
½ÇÇè 28 Tyrosinase È¿¼ÒÀÇ »ýÈ­ÇÐÀû Ư¼º 192
½ÇÇè 28 Production of fusion protein (cloned genes) in E. coli 196
½ÇÇè 29 SDS-Polyacrylamide Gel electrophoresis 201
½ÇÇè 30 Western blotting 209
½ÇÇè 31 Translation of mRNA 218
½ÇÇè 32 Immunoprecipitation of target proteins 223
½ÇÇè 33 ELISA(Enzyme-Linked ImmunoSorvent Assay) ¹ÝÀÀ-È¿¼Ò¿¡°áÇÕÇÑ ¸é¿ªÈíÂøÁ¦ ºÐ¼®¹ý 229
½ÇÇè 34 plaque purification of Bacteriophage ¥ë 233
½ÇÇè 35 Single plaqueÀ¸·ÎºÎÅÍ bacteriophage ¥ëstockÀÇ Á¦Á¶ 237
½ÇÇè 36 Small-scale bacteriophage ¥ë liquid culture 241
½ÇÇè 37 Bacteriophage ¥ëDNAÀÇ Á¦Á¶ 244
½ÇÇè 38 bacteriophage ¥ëDNAÀÇ Á¦ÇÑÈ¿¼Ò¿¡ ÀÇÇÑ digestion 248
½ÇÇè 39 Bacteriophage ¥ëDNA armsÀÇ Á¤Á¦ 251
½ÇÇè 40 Bacteriophage ¥ëvectorÀÇ alkaline phosphatase È¿¼Ò·ÎÀÇ Ã³¸® 255
½ÇÇè 41 Bacteriophage ¥ëÀÇ foreign DNA fragment¿ÍÀÇ ligation and packaging 259

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ºÎ·Ï 2 ºÐÀÚ»ý¹°ÇÐ ¹°ÁúÀÇ ´ÜÀ§µé 276
ºÎ·Ï 3 ¿ÏÃæ¿ë¾× 277
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ºÎ·Ï 5 Á¤»óÀÎÀÇ ¼Òº¯ ¼ººÐÇ¥(24½Ã°£´¢) 281
ºÎ·Ï 6 À¯¿ëÇÑ Web Sites 282
ºÎ·Ï 7 »ý¸í°øÇÐ Á¤º¸ Web Sites 285

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