½ÇÇè 1 ¹ÚÅ׸®¾Æ ¹è¾ç ¹èÁö 32½ÇÇè 2 Pure ¹ÚÅ׸®¾ÆÀÇ ºÐ¸® ¹× ¹è¾ç 41½ÇÇè 3 ¼¼±Õ ¼öÀÇ ÃøÁ¤(ÆòÆÇ °è¼ö¹ý, Plate count technique) 47½ÇÇè 4 ¹ÚÅ׸®¾Æ ¼¼Æ÷ÀÇ ¼ºÀå ¹× ±ÕÁÖ(strain) º¸°ü¹ý 53½ÇÇè 5 Á¢ÇÕ(conjugation) 61½ÇÇè 6 ¹ÚÅ׸®¾Æ ¼¼Æ÷·ÎºÎÅÍ plasmid DNAÀÇ extraction°ú purification 64½ÇÇè 7 ÇÙ»ê°ú ´Ü¹éÁúÀÇ ³óµµ ÃøÁ¤ 73½ÇÇè 8 Agarose gel electrophoresis 77½ÇÇè 9 Agarose gel·ÎºÎÅÍ fragmented DNAÀÇ recovery ¹× Á¤Á¦ 83½ÇÇè 10 Plasmid vector¸¦ ÀÌ¿ëÇÑ subcloning 87½ÇÇè 11 Recombinant Plasmid DNAÀÇ ÇüÁúÀüȯ 94½ÇÇè 12 ÇüÁúÀüȯüÀÇ °íµî»ý¹°Ã¼·ÎÀÇ ÇüÁúÀüȯ ¹× ºÐ¼® 100½ÇÇè 13 ¥â-galactosidase enzymeÀÇ induction 113½ÇÇè 14 Recombinant plasmid DNAÀÇ Å½Áö¿Í Á¦ÇÑÈ¿¼Ò Áöµµ ÀÛ¼º 117½ÇÇè 15 ÁøÇÙ»ý¹°·ÎºÎÅÍ Genome DNAÀÇ ÃßÃâ 123½ÇÇè 16 Eukaryotic DNAÀÇ partial restriction enzyme digestion 127½ÇÇè 17 Southern Hybridization 130½ÇÇè 18 In situ hybridization 136½ÇÇè 19 ¹ýÀÇÇÐÀû Ãø¸é¿¡¼ÀÇ fingerprinting 143½ÇÇè 20 ÁßÇÕÈ¿¼Ò ¿¬¼â¹ÝÀÀ(PCR, Polymerase Chain Reaction) 151½ÇÇè 21 UV¿¡ ÀÇÇÑ DNA ¼Õ»ó ¹× ±¤È¸º¹ 155½ÇÇè 22 °íµî»ý¹° Á¶Á÷À¸·ÎºÎÅÍ total RNAÀÇ extraction 159½ÇÇè 23 mRNA(poly(A)+)ÀÇ Purification and selection 168½ÇÇè 24 Northern hybridization 172½ÇÇè 25 Dot and slot hybridization of RNA 180½ÇÇè 26 Tyrosinase ´Ü¹éÁúÀÇ ÃßÃâ ¹× Á¤Á¦ 185½ÇÇè 28 Tyrosinase È¿¼ÒÀÇ »ýÈÇÐÀû Ư¼º 192½ÇÇè 28 Production of fusion protein (cloned genes) in E. coli 196½ÇÇè 29 SDS-Polyacrylamide Gel electrophoresis 201½ÇÇè 30 Western blotting 209½ÇÇè 31 Translation of mRNA 218½ÇÇè 32 Immunoprecipitation of target proteins 223½ÇÇè 33 ELISA(Enzyme-Linked ImmunoSorvent Assay) ¹ÝÀÀ-È¿¼Ò¿¡°áÇÕÇÑ ¸é¿ªÈíÂøÁ¦ ºÐ¼®¹ý 229½ÇÇè 34 plaque purification of Bacteriophage ¥ë 233½ÇÇè 35 Single plaqueÀ¸·ÎºÎÅÍ bacteriophage ¥ëstockÀÇ Á¦Á¶ 237½ÇÇè 36 Small-scale bacteriophage ¥ë liquid culture 241½ÇÇè 37 Bacteriophage ¥ëDNAÀÇ Á¦Á¶ 244½ÇÇè 38 bacteriophage ¥ëDNAÀÇ Á¦ÇÑÈ¿¼Ò¿¡ ÀÇÇÑ digestion 248½ÇÇè 39 Bacteriophage ¥ëDNA armsÀÇ Á¤Á¦ 251½ÇÇè 40 Bacteriophage ¥ëvectorÀÇ alkaline phosphatase È¿¼Ò·ÎÀÇ Ã³¸® 255½ÇÇè 41 Bacteriophage ¥ëÀÇ foreign DNA fragment¿ÍÀÇ ligation and packaging 259ºÎ·Ï 1 ºÐÀÚ»ý¹°ÇÐ °ü·Ã ¿ë¾× Á¦Á¶ 265ºÎ·Ï 2 ºÐÀÚ»ý¹°ÇÐ ¹°ÁúÀÇ ´ÜÀ§µé 276ºÎ·Ï 3 ¿ÏÃæ¿ë¾× 277ºÎ·Ï 4 ¾Ð·ÂÀ» ¿Âµµ·Î ȯ»êÇÏ´Â ¹ý 280ºÎ·Ï 5 Á¤»óÀÎÀÇ ¼Òº¯ ¼ººÐÇ¥(24½Ã°£´¢) 281ºÎ·Ï 6 À¯¿ëÇÑ Web Sites 282ºÎ·Ï 7 »ý¸í°øÇÐ Á¤º¸ Web Sites 285