The eighth edition of this well-recognised textbook preserves the tradition of excellence created by previous editions. Comprehensive and authoritative, the book explores all of the topics crucial to an understanding of gene cloning in an approachable way. An easy-to-follow and user-friendly layout is presented in full-colour throughout the volume, making it simple to absorb the clear and accessible material contained within.
Gene Cloning and DNA Analysis: An Introduction, Eighth Edition contains updated and extended coverage of gene editing strategies like CRISPR/Cas, rewritten chapters on DNA sequencing and genome studies, as well as new material on real-time PCR and typing of human disease mutations. Over 250 full-colour illustrations are included to bring to life the comprehensive content. The book also covers topics like:
¡Ü The strategies used by researchers and industry practitioners to assemble genome sequences
¡Ü Next-Generation Sequencing methods and descriptions of their applications in studying genomes and transcriptomes
¡Ü Includes the use and application of gene editing strategies
¡Ü Interbreeding between Neanderthals and Homo sapiens
Part I The Basic Principles of Gene Cloning and DNA Analysis
Chapter 1 Why Gene Cloning and DNA Analysis are Important
Chapter 2 Vectors for Gene Cloning: Plasmids and Bacteriophages
Chapter 3 Purification of DNA from Living Cells
Chapter 4 Manipulation of Purified DNA
Chapter 5 Introduction of DNA into Living Cells
Chapter 6 Cloning Vectors for E. coli
Chapter 7 Cloning Vectors for Eukaryotes
Chapter 8 How to Obtain a Clone of a Specific Gene
Chapter 9 The Polymerase Chain Reaction
Part II The Applications of Gene Cloning and DNA Analysis in Research 187
Chapter 10 Sequencing Genes and Genomes
Chapter 11 Studying Gene Expression and Function
Chapter 12 Studying Genomes
Chapter 13 Studying Transcriptomes and Proteomes
Part III The Applications of Gene Cloning and DNA Analysis in Biotechnology
Chapter 14 Production of Protein from Cloned Genes
Chapter 15 Gene Cloning and DNA Analysis in Medicine
Chapter 16 Gene Cloning and DNA Analysis in Agriculture
Chapter 17 Gene Cloning and DNA Analysis in Forensic Science and Archaeology
Glossary
Index
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