THE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS, CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES
Freshney’s Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications in the field of cell and tissue culture. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much more.
The eighth edition contains extensively revised material that reflects the latest techniques and emerging applications in cell culture, such as the use of CRISPR/Cas9 for gene editing and the adoption of chemically defined conditions for stem cell culture. A brand-new chapter examines the origin and evolution of cell lines, joined by a dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice. Throughout the book, updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing. This landmark text:
Part I UNDERSTANDING CELL CULTURE
Chapter 1 Introduction
Chapter 2 Biology of Cultured Cells
Chapter 3 Origin and Evolution of Cultured Cells
Chapter 4 Laboratory Design and Layout
Part II LABORATORY AND REGULATORY REQUIREMENTS
Chapter 5 Equipment and Materials
Chapter 6 Safety and Bioethics
Chapter 7 Reproducibility and Good Cell Culture Practice
Part III MEDIUM AND SUBSTRATE REQUIREMENTS
Chapter 8 Culture Vessels and Substrates
Chapter 9 Defined Media and Supplements
Chapter 10 Serum-free Media
Chapter 11 Preparation and Sterilization
Part IV HANDLING CULTURES
Chapter 12 Aseptic Technique
Chapter 13 Primary Culture
Chapter 14 Subculture and Cell Lines
Chapter 15 Cryopreservation and Banking
Part V VALIDATION AND CHARACTERIZATION
Chapter 16 Microbial Contamination
Chapter 17 Cell Line Misidentification and Authenticatio
Chapter 18 Cell Line Characterization
Chapter 19 Quantitation and Growth KineticsChapter
Part VI PHYSICAL AND GENETIC MANIPULATION
Chapter 20 Cell Cloning and Selection
Chapter 21 Cell Separation and Sorting
Chapter 22 Genetic Modification and Immortalization
Part VII STEM CELLS AND DIFFERENTIATED CELLS
Chapter 23 Culture of Stem Cells
Chapter 24 Culture of Specific Cell Types
Chapter 25 Culture of Tumor Cells
Chapter 26 Differentiation
Part VIII MODEL ENVIRONMENTS AND APPLICATIONS
Chapter 27 Three-dimensional Culture
Chapter 28 Scale-up and Automation
Chapter 29 Toxicity Testing
Part IX TEACHING AND TROUBLESHOOTING
Chapter 30 Training
Chapter 31 Problem Solving
Chapter 32 In Conclusion
{교재 사용시 강의 자료 문의 바랍니다.}
상품정보고시
| 제품명 |
Culture of Animal Cells 8E |
| 판매가격 |
65,000원 |
| 제조사 |
Wiley |
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