Introductory Unit
Introductory Lecture - Introduction to the Biochemical Laboratory
Introductory Lab 1 - Basic Biochemical Techniques I: Pipette Calibration and Solution Preparation
Introductory Lab 2 - Basic Techniques II: Absorbance Spectroscopy and Protein Concentration Determinations
Part I - Nculeic Acids & Cloning
Unit 1
Lecutre 1 - DNA Isolation
Lab 1.1 - Media preparation; Bact
Lab 1.2 - Agarose Gel Electrophoresis
Unit 2
Lecuture 2 - Construction of Recombinant Plasmids
Lab 2.1 - Extraction and Cleanup of DNA Bands from Agrose Gels, Quantification of Yields and Ligation of myo-3 Hind lll DNA Insert Fragment into Linearized ?-gal Plasmic DNA
Unit 3
Lecture 3 - The Polymerase Chain Reactin
Lab 3.1 - Polymerase Chain Reaction Test for myo-3 Gene Insert Orientation
Unit 4
Lecture 4 - Transcription of Genomic DNA & Analysis of the Resulting mRNAs
Unit 5
Lecture 5 - Transformation and Gene Expression
Lab 5.1 - Preparation of Fresh Transformation - Competent Cells
Lab 5.2 - Colony Immunoblotting to Screen for Transformants
Unit 6
Lecture 6 - Analysis of DNA or RNA by Duplex Hybridization: DNA Isolation, Labeling, and Probing
Lab 6.1 - Labeling of DNA and Probe Construction from Cloned C. elegans myo-3 Gene; Quantitation of DNA Concentration
Lab 6.2 - Isolation of C. elegans Genomic DNA, Quantation of DNA Concetration, and Digestion to Extract the myo-3 Gene
Lab 6.3 - Southern Blotting
Part 2 - Protein Purification
Unit 7
Lecture 7 - Protein Purification
Lab 7.1 - The Protein Purifier: A Learning Aid from Pharmacia
Lab 7.2 - Induction and Purification of B-Galactosidase Fusion Protein from Bacteria
Lab 7.3 - Gel Filtration of Molecular Weight Standards and Protein Fractionation
Lab 7.4 - Mciroplate B-Glactosidase Assay to Determine Fractions Containing Fusion Protein; MW Determination
Lab 7.5 - Ion Exchange Column Chromatography
Lab 7.6 - Affinity Chromatography and Microplate b-Glactosidase Assays to Construct an Enzyme Purification Table
Unit 8
Lecture 8 - Discontinuous Gel Electrophoresis, Protein Mobilities and Apparent Size Determination
Lab 8.1 - Discontinuous SDS Gel Electrophoresis
Unit 9
Lecture 9 - Immunochemical Techniques
Lab 9.1 - Western Blotting
Unit 10
Lecture 10 - Combinatorial Biochemical Technology
Appendices
Index