Preface

1. Gene Manipulation in the Post-Genomics Era

Part I: Fundamental Techniques of Gene Manipulation:
2. Basic Techniques
3. Cutting and Joining DNA Molecules
4. Basic Biology of Plasmid and Phage Vectors
5. Cosmids, Phasmids and Other Advanced Vectors
6. Gene Cloning Strategies
7. Sequencing Genes and Short Stretches of DNA
8. Changing Genes: Site-directed Mutagenesis and Protein Engineering
9. Bioinformatics

Part II: Manipulating DNA in Microbes, Plants and Animals:
10. Cloning in Bacteria Other Than Escherichia coli
11. Cloning in Saccharomyces cerevisiaea and Other Fungi
12. Gene Transfer to Animal Cells
13. Genetic Manipulation of Animals
14. Gene Transfer to Plants
15. Advanced Transgenic Technology

Part III: Genome Analysis, Genomics and Beyond:
16. The Organization and Structure of Genomes
17. Mapping and Sequencing Genomes
18. Comparative Genomics
19. Large-Scale Mutagenesis and Interference
20. Analysis of the Transcriptome
21. Proteomics I - Expression Analysis and Characterization of Proteins
22. Proteomics II - Analysis of Protein Structures
23. Proteomics III - Protein Interactions
24. Metabolomics and Global Biochemical Networks

Part IV: Applications of Gene Manipulation and Genomics:
25. Applications of Genomics: Understanding the Basis of Polygenic Disorders and Identifying Quantitative Trait Loci
26. Applications of Recombinant DNA Technology

Index