Project I:  Genomic DNA Isolation and Analysis

Module I.1   Mouse Genomic DNA Isolation and Analysis

Module I.2   Southern Blot Experiment to Detect Gene Specific Sequences in Genomic DNA

Module I.3   PCR Amplify a Specific Gene from Mouse Genomic DNA

Project II:  Genomic Cloning, DNA Sequencing and Bioinformatics

Module II.1   Prepare Mouse Genomic DNA for Cloning, Production and Titering of a Lambda Phage Genomic Library

Module II.2   Screen a Lambda Phage Library to Detect Clones of a Specific Gene

Module II.3   PCR Subclone into a Plasmid Vector, Propagate in Bacterial Cells, and Prepare Purified Recombinant Plasmid DNA

Module II.4   Dideoxy Thermal Cycle DNA Sequencing of a Cloned Gene

Module II.5   Bioinformatic Analysis of the DNA Sequence Data

Project III:  Gene Expression Analysis:  RNA Isolation, Northern Blot, and Reverse Transcriptase-Polymerase Chain Reaction

Module III.1   Isolation and Analysis of Total RNA from Mouse Liver

Module III.2   Northern Blot Experiment to Detect a Specific Gene Transcript

Module III.3   Reverse Transcriptase-Polymerase Chain Reaction Amplification of a Specific Gene Transcript

Project IV:  cDNA Cloning and cDNA  Library Analysis

Module IV.1   Construction of a cDNA Library and cDNA Library Analysis

Module IV.2   Characterization and Screening of a cDNA Library Using the Polymerase Chain Reaction

Appendix I:     Available Kits and Componenets

Appendix II:   General Molecular Biology Laboratory Set Up

Appendix III:  Marker Maps and Sizes

Appendix IV:  Alternate and Additional Protocols

Appendix V:   Other Useful Protocols from RT-PCR Reactions

Appendix VI:  Useful Calculations

Appendix VII: References, Helpful Books, and Additional Resources