394 pagesTrim Size 7 1/2 X 9 1/4 inCopyright 2010Handbook, Reference===============================Protein Determination Phosphopeptide Mapping: A Basic Protocol Coupling of Fluorescent Tags to Proteins Radioiodination of Proteins and Peptides Free-Flow Electrophoresis Gel-Based Proteomics: High-Resolution Two-Dimensional Gel Electrophoresis of Proteins. Isoelectric Focusing and Nonequalibrium pH Gradient Electrophoresis. High-Resolution Two-Dimensional Electrophoresis with Immobilized pH Gradients for Proteome Analysis Two-Dimensional Difference Gel Electrophoresis: Application for the Analysis of Differential Protein Expression in Multiple Biological Samples Affinity Electrophoresis for Studies of Biospecific Interations: High-Resolution Two-Dimensional Affinity Electrophoresis for Separation of Hapten-Specific Polyclonal Antibodies into Monoclonal Antibodies in Murine Blood Plasma Image Analysis and Quantitation Fluorescence Detection of Proteins in Gels Using SYPRO Dyes Autoradiography and Fluorography: Film-Based Techniques for Imaging Radioactivity in Flat Samples Two-Dimensional Gel Profiling of Posttanslationally Modified Proteins by in vivo Isotope Labeling Immunoprecipitation of Proteins under Nondenaturing Conditions Nondenaturing Polyacrylamide Gel Electrophoresis as a Method for Studying Protein Interations: Applications in the Analysis of Mitochrondrial Oxidative Phosphorylation Complexes Affinity purification with Natural Immobilized Ligands Analysis of Protein-Protein Interactions by Chemical Cross-Linking Peroxisomal Targeting as a Tool to Assess Protein-Protein Interactions Biomolecular Interaction Analysis Mass Spectrometry Blot Overlays with P-Labedled GST-Ras Fusion Proteins: Application to Mapping Protein-Protein Interaction Sites Ligand Blot Overlay Assay: Detetion of Ca+2- and Small GTP-Binding Proteins Modular Scale Yeast Two-Hybrid Screening Chromophore-Assisted Laser Inactivation of Proteins by Antibodies Labeled with Malachite Green Chromatin Immunoprecipitation (ChIP) Gel Mobility Shift Assay DNA Affinity Chromatography of Transcription Factors: The Oligaonucleotide Trapping Approach Protein Degradation Methods: Chaperone-Mediated Autophagy Methods in Protein Ubiquitination Protein Identification and Sequencing by Mass Spectrometry Proteome Specific Sample Preparation Methods for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry In-Gel Digestion of Protein Spots for Mass Spectrometry Peptide Sequencing by Tandem Mass Spectrometry Direct Database Searing Using Tandem Mass Spectra of Peptides Identification of Proteins from Organisms with Unsequenced Genomes by Tandem Mass Spectrometry and Sequence-Similarity Database Searching Tools Identification of Protein Phosphorylation Sites by Mass Spectrometry Analysis of Carbohydrates/Glycoproteins by Mass Spectrometry Stable Isotope Labeling by Amindo Acids in Cell Culture for Quantitative Proteomics Site-Specific, Stable Isotopic Labeling of Cysteinyl Pepties in Complex Peptide Mixtures Protein Hydrogen Exchange measured by Electrospry Ionization Mass Spectrometry Nongel Based Proteomics: Selective Reversed-Phase Chromatographic Isolation of Methionine-Containing Peptides from Complex Peptide Mixtures Mass Spectrometry in Noncovalent Protein Interactions and Protein Assemblies
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